旗赛生物科技(武汉)有限公司
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人弥漫大B淋巴瘤细胞 U-2932(STR鉴定正确)
细胞照片
细胞详细信息
细胞中文名称 | 人弥漫大B淋巴瘤细胞 |
细胞英文名称 | U-2932 |
细胞别称 | U2932 |
建系背景 | established in 1996 from the ascites of a 29-year-old woman with diffuse large B cell lymphoma, who 16 years earlier was diagnosed with advanced stage Hodgkin lymphoma, and relapsed several times after multiple chemo- and radiotharapy regimens to complete remissions; cells were described in the literature to overexpress BCL2, BCL6 and p53; assigned to ABC-like lymphoma subtype (activated B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq). Also, please note: the cell line U-2932 harbours two distinct subpopulations as visible by surface staining of CD19, CD20, and CD38 (see ref 18133); continuous monitoring of subpopulations is advised to prevent accidental loss during passaging |
组织来源 | 腹水 |
生长特性 | 悬浮细胞 |
细胞形态 | 淋巴母细胞样,圆形,单个细胞,少数聚团 |
细胞培养条件 | |
空气,95%;CO2,5%;温度:37℃ | |
推荐冻存条件 | 30% 基础培养基+60%FBS+10%DMSO |
液氮保存 | |
推荐传代比例 | 1:3--1:4 |
推荐换液频率 | 2-3次/周 |
生物安全等级 | 1 |
成瘤性 | |
注意事项: | 本司产品仅用于科研,不用于临床诊断和治疗 |