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永生化人脑微血管内皮细胞 hCMEC/D3(STR鉴定正确)
细胞照片
细胞详细信息
细胞中文名称 | 永生化人脑微血管内皮细胞 |
细胞英文名称 | hCMEC/D3 |
细胞别称 | HCMEC/D3; CMEC/D3; human Cortical Microvessels Endothelial Cells/D3 |
建系背景 | The hCMEC/D3 cell line was derived from human temporal lobe microvessels isolated from tissue excised during surgery for control of epilepsy. The primary isolate was enriched in cerebral endothelial cells (CECs). In the first passage, cells were sequentially immortalized by lentiviral vector transduction with the catalytic subunit of human telomerase (hTERT) and SV40 large T antigen, following which CEC were selectively isolated by limited dilution cloning, and clones were extensively characterized for brain endothelial phenotype. This brain microvascular endothelial cell line represents one such model of the human BBB that can be easily grown and is amenable to cellular and molecular studies on pathological and drug transport mechanisms with relevance to the central nervous system (CNS). |
组织来源 | 脑微血管 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
细胞培养条件 | ECM内皮专用培养基 |
空气,95%;CO2,5%;温度:37℃ | |
推荐冻存条件 | 30% 基础培养基+60%FBS+10%DMSO |
液氮保存 | |
推荐传代比例 | 1:3--1:5 |
推荐换液频率 | 2-3次/周 |
生物安全等级 | 2 |
成瘤性 | |
注意事项: | 本司产品仅用于科研,不用于临床诊断和治疗 |